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Furthermore, in Calcr Cre rats injected with adeno-associated virus (AAV)-DIO-hM3Dq-mCherry in the AP, essentially all mCherry-identified AP Calcr Cre neurons contained CALCR but not GLP1R immunoreactivity (we lack a reliable reporter allele for Calcr Cre in rats

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Log-rank (Mantel-Cox) test was used for survival analysis ( n = 60 in zebrafish experiment, n = 10 in mice experiment)